Improved efficiency for primer extension by using a long, highly-labeled primer generated from immobilized single-stranded DNA templates.

AUTOR(ES)

Flouriot, G

RESUMO

Primer extension is one of the most common methods used to measure the amount and size of RNAs. We demonstrate that the sensitivity and the specificity of this method are improved considerably by using a highly-labeled single-stranded DNA generated from a biotinylated single-stranded DNA template, as a long specific primer in the reverse transcription reaction. This new approach allows the detection of transcripts with a low expression level from microgram quantities of total RNA.

Documentos Relacionados

• Fingerprinting of diverse species with DNA probes generated from immobilized single-stranded DNA templates.
• A New Form of DNA Polymerase III and a Copolymerase Replicate a Long, Single-Stranded Primer-Template†
• Transcription of tRNA genes in vivo: single-stranded compared to double-stranded templates.
• Sequence-specific arrest of primer extension on single-stranded DNA by an oligonucleotide-minor groove binder conjugate.
• A Role for Single-Stranded Templates in Cell-Free Adeno-Associated Virus DNA Replication