Improved Secretion of Native Human Insulin-Like Growth Factor 1 from gas1 Mutant Saccharomyces cerevisiae Cells
AUTOR(ES)
Vai, Marina
FONTE
American Society for Microbiology
RESUMO
We studied the secretion of recombinant human insulin-like growth factor 1 (rhIGF-1) from transformed yeast cells. The hIGF-1 gene was fused to the mating factor α prepro- leader sequence under the control of the constitutive ACT1 promoter. We found that the inactivation of the GAS1 gene in the host strain led to a supersecretory phenotype yielding a considerable increase, from 8 to 55 mg/liter, in rhIGF-1 production.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=92485Documentos Relacionados
- Isolation of the human insulin-like growth factor genes: insulin-like growth factor II and insulin genes are contiguous.
- Differential regulation of insulin-like growth factor binding protein secretion from human decidual cells by IGF-I, insulin, and relaxin.
- Human pituitary and placental hormones control human insulin-like growth factor II secretion in human granulosa cells.
- Insulin-like growth factor-1 stimulation of lymphopoiesis.
- The receptor for insulin-like growth factor II mediates an insulin-like response.