In situ detection of protein-DNA interactions in filamentous fungi by in vivo footprinting.
AUTOR(ES)
Wolschek, M F
RESUMO
The method described here allows the detection of protein-DNA interactions in vivo in filamentous fungi. We outline culture conditions and conditions of in vivo methylation that permit uniform modification of all cells in an apically growing, non-uniform organism, and subsequent visualization of protected areas by ligation-mediated PCR.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=147760Documentos Relacionados
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