In Vitro Analysis of the General Properties and Junctional Receptor Characteristics of Skeletal Muscle Membranes. Isolation, Purification, and Partial Characterization of Sarcolemmal Fragments*

AUTOR(ES)

Festoff, Barry W.

RESUMO

Muscle membranes were partially purified from rat leg muscles. Externally oriented membrane functions were used to monitor and characterize the resulting membrane fractions. Na+K+-stimulated Mg++-adenosinetriphosphatase, acetylcholinesterase, and cholinergic receptor activities are present and enriched in the density-gradient subfractions of crude sarcolemma when compared with the first pellet. The physical separation of the cholinesterase and receptor activities on the gradient subfractions is demonstrated.

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