In vitro DNA synthesis by an alpha-like DNA polymerase bound to replicating simian virus 40 chromosomes.
AUTOR(ES)
Waqar, M A
RESUMO
Simian virus 40 chromosomes carry out replicative DNA synthesis in vitro which is sensitive to aphidicolin and to N-ethylmaleimide, resistant to 2',3'-dideoxythymidine-5'-triphosphate, and proportional to the amount of chromosome-associated alpha-like polymerase. Thus, an alpha-like DNA polymerase (alpha polymerase or delta polymerase) is responsible for in vitro DNA synthesis.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=255347Documentos Relacionados
- DNA synthesis by partially purified replicating simian virus 40 chromosomes.
- Aphidicolin inhibits DNA polymerase II of Escherichia coli, an alpha-like DNA polymerase.
- Polymerization activity of an alpha-like DNA polymerase requires a conserved 3'-5' exonuclease active site.
- Simian Virus 40 DNA Replication in Isolated Replicating Viral Chromosomes
- The hyperthermophilic archaeon Pyrodictium occultum has two alpha-like DNA polymerases.
