In vitro maturation of mesencephalic dopaminergic neurons from mouse embryos is enhanced in presence of their striatal target cells.
AUTOR(ES)
Prochiantz, A
RESUMO
Long-term survival of mesencephalic and striatal cells from mouse embryos in dissociated primary cultures is described. Catecholaminergic neurons in mesencephalic culutres were identified histochemically and by measuring [3H]dopamine uptake and synthesis from [3H]tyrosine. According to experiments using specific inhibitors of catecholamine uptake, at least two-thirds of the catecholaminergic neurons are dopaminergic. These neurons differentiated whether or not striatal target cells were present, but striatal cells stimulated the development of the dopaminergic neurons. [3H]Dopamine uptake was increased by at least 2-fold regardless of the age of the cocultures (4-15 days). Enhanced [3H]dopamine synthesis was also observed (at least 2-fold) at later times (12-15 days).
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=413148Documentos Relacionados
- Target neuron-specific process formation by embryonic mesencephalic dopamine neurons in vitro.
- 5,7-Dihydroxytryptamine identifies living dopaminergic neurons in mesencephalic cultures.
- Nurr1 is essential for the induction of the dopaminergic phenotype and the survival of ventral mesencephalic late dopaminergic precursor neurons
- Selective loss of dopaminergic nigro-striatal neurons in brains of Atm-deficient mice
- A homeodomain gene Ptx3 has highly restricted brain expression in mesencephalic dopaminergic neurons