In vitro packaging of bacteriophage T7 DNA requires ATP.
AUTOR(ES)
Masker, W E
RESUMO
Removal of nucleoside triphosphates from extracts prepared from bacteriophage T7-infected Escherichia coli results in a stringent requirement for added ATP to form infective phage particles by in vitro packaging of bacteriophage T7 DNA. Optimal packaging efficiency was achieved at a concentration of about 1.25 mM. Other nucleoside triphosphates could be substituted for ATP, but none of the common nucleoside triphosphates was as effective as ATP in promoting in vitro encapsulation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=256132Documentos Relacionados
- Packaging and Maturation of DNA of Bacteriophage T7 In Vitro
- DNA packaging in vitro by an isolated bacteriophage T7 procapsid.
- In Vitro Packaging of UV Radiation-Damaged DNA from Bacteriophage T7
- In Vitro Packaging of UV Radiation-Damaged DNA from Bacteriophage T7
- Gene 2 protein of bacteriophage T7: purification and requirement for packaging of T7 DNA in vitro.
