In vitro synthesis of late bacteriophage phi 29 RNA.
AUTOR(ES)
Holder, R D
RESUMO
A crude P-100 fraction prepared from Bacillus subtilis 21 min after infection with wild-type phage phi 29 supported the in vitro synthesis of late phi 29 RNA by added RNA polymerase. Synthesis of late RNA was also detected when purified phi 29 DNA was transcribed by RNA polymerase in the presence of an S-150 fraction obtained by lysis of phi 29-infected cells in the presence of 1 M NaCl. Late phi 29 RNA was not synthesized when either the P-100 or the S-150 fraction was prepared from cultures infected with phi 29 having a mutation in gene 4.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=256545Documentos Relacionados
- In vitro synthesis of T4 late messenger RNA.
- In vitro replication of bacteriophage phi 29 DNA.
- In vitro packaging and replication of individual genomic segments of bacteriophage phi 6 RNA.
- In vitro assembly of the Bacillus subtilis bacteriophage phi 29.
- Bacteriophage Nf DNA region controlling late transcription: structural and functional homology with bacteriophage phi 29.
