In Vitro Synthesis of Ureidohomoserine by an Enzyme from Jack Bean (Canavalia ensiformis) Leaves
AUTOR(ES)
O'Neal, Thomas Denny
RESUMO
An enzyme was extensively purified from jack bean leaves (Canavalia ensiformis L.) which produced o-ureidohomoserine from l-canaline and carbamyl phosphate. The most highly purified preparations catalyzed both this reaction and citrulline synthesis from ornithine and carbamyl phosphate, and the ratio of the two activities remained nearly constant during purification. When hydrated jack bean seeds were the enzyme source, ornithine carbamyltransferase (EC 2.1.3.3) activity was high but synthesis of ureidohomoserine was barely detectable. Both ornithine carbamyltransferase and the ureidohomoserine synthesizing enzyme had similar Km values for carbamyl phosphate. The purification data suggest that one enzyme may catalyze both reactions in jack bean leaves.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=541749Documentos Relacionados
- Cloning, expression, and crystallization of jack bean (Canavalia ensiformis) canavalin.
- The three-dimensional structure of canavalin from jack bean (Canavalia ensiformis).
- Large Effects of Small Water Deficits on Chlorophyll Accumulation and Ribonucleic Acid Synthesis in Etiolated Leaves of Jack Bean (Canavalia ensiformis [L.] DC.) 1
- Avaliação de extratos das folhas e sementes de feijão-de-porco (Canavalia ensiformis) como bioerbicidas pós-emergentes e identificação de aleloquímicos via cromatografia líquida de alta eficiência (HPLC)
- Ultrastructural Effects of Water Stress on Chloroplast Development in Jack Bean (Canavalia ensiformis [L.] DC) 1