In vitro-synthesized hepatitis delta virus RNA initiates genome replication in cultured cells.
AUTOR(ES)
Glenn, J S
RESUMO
Monomers of the genomic strand of hepatitis delta virus RNA were transcribed in vitro and then delivered to NIH 3T3 fibroblasts by using a liposome fusion technique. After 7 days, genome replication was detected, but only in fibroblasts that stably expressed the delta antigen. Sequence analysis of the replicated products identified them as faithful copies of the hepatitis delta virus genome found in virions.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=249503Documentos Relacionados
- The In Vitro-Synthesized RNA from a cDNA Clone of Hepatitis E Virus Is Infectious
- In vitro-synthesized adenovirus 2 messenger RNA precursors are accurately spliced by nuclear extracts.
- Genome organization of RNA tumor viruses II. Physical maps of in vitro-synthesized Moloney murine leukemia virus double-stranded DNA by restriction endonucleases.
- Post-Translational Proteolytic Cleavage of In Vitro-Synthesized Turnip Yellow Mosaic Virus RNA-Coded High-Molecular-Weight Proteins
- ATP is required for initiation of poliovirus RNA synthesis in vitro: demonstration of tyrosine-phosphate linkage between in vitro-synthesized RNA and genome-linked protein.
