In vitro transcription of the avian retrovirus genome by the alpha form of the viral RNA-directed DNA polymerase.
AUTOR(ES)
Collett, M S
RESUMO
The nature of transcription of the avian retrovirus RNA genome by the alpha form of the viral RNA-directed DNA polymerase has been investigated. Transcription was most efficient when Mn2+ was provided as the divalent metal ion. The patterns of DNA transcription using 70S RNA, 35S RNA-tRNAtrp, or 35S RNA-oligo(dT)12-18 template-primer complexes by the alpha DNA polymerase were essentially identical to those obtained using the alphabeta form. The alpha DNA polymerase appears to be deficient in the synthesis of true duplex DNA but is able to synthesize hairpin-structured DNA initiated at the 5' terminus of the viral genome on the tRNAtrp primer molecule.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=515984Documentos Relacionados
- Unwinding-like activity associated with avian retrovirus RNA-directed DNA polymerase.
- Primer recognition by avian myeloblastosis virus RNA-directed DNA polymerase.
- In vitro transcription of the avian oncornavirus genome by the RNA-directed DNA polymerase: effect of actinomycin D on the extent of transcription.
- Human placentas contain a specific inhibitor of RNA-directed DNA polymerase.
- DNA-DEPENDENT RNA-DIRECTED PROTEIN SYNTHESIS in vitro, I. EXTENT OF GENOME TRANSCRIPTION*
