In vivo cloning of Erwinia carotovora genes involved in the catabolism of hexuronates.
AUTOR(ES)
Van Gijsegem, F
RESUMO
Using the RP4::mini-Mu pULB113 plasmid, an RP4 derivative carrying a deleted Mu prophage which allows the plasmid to pick up any chromosomal DNA segment to form R' plasmids, we cloned all of the genes of Erwinia carotovora involved in the catabolism of the hexuronates and in the transport of these substrates. With the R' plasmids we isolated, we performed complementation analysis and found that, in the Erwinia carotovora strain we used, the genes involved in the catabolism of the hexuronates are clustered in four regions of the chromosome. This genetic organization is compared with that of Escherichia coli K-12.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=217595Documentos Relacionados
- Isolation and characterization of Erwinia chrysanthemi mutants defective in degradation of hexuronates.
- Cloning and expression in Escherichia coli of pectinase genes of Erwinia carotovora subsp. carotovora.
- In vivo cloning of the pectate lyase and cellulase genes of Erwinia chrysanthemi
- Two Genomic Regions Involved in Catechol Siderophore Production by Erwinia carotovora
- Comparison of pectic enzymes produced by Erwinia chrysanthemi, Erwinia carotovora subsp. carotovora, and Erwinia carotovora subsp. atroseptica.