In vivo cloning of the pectate lyase and cellulase genes of Erwinia chrysanthemi
AUTOR(ES)
Gijsegem, Frédérique van
RESUMO
Using an RP4 plasmid which carries a mini-Mu prophage which allows it to integrate spontaneously random pieces of its host chromosome, we cloned in vivo at least some of the pectate lyase and cellulase genes of the Erwinia chrysanthemi strain B374. The RP4-prime plasmids were used to localize the cloned genes on the B374 chromosome by co-transposition mapping and to subclone most of the genes in a classic high copy number plasmid vector.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=554257Documentos Relacionados
- Molecular cloning of pectate lyase genes from Erwinia chrysanthemi and their expression in Escherichia coli.
- Molecular cloning in Escherichia coli of Erwinia chrysanthemi genes encoding multiple forms of pectate lyase.
- Molecular cloning of Erwinia chrysanthemi pectinase and cellulase structural genes
- Pectate lyase gene regulatory mutants of Erwinia chrysanthemi.
- Evidence of homology between the pectate lyase-encoding pelB and pelC genes in Erwinia chrysanthemi.