In vivo evidence for the involvement of anionic phospholipids in initiation of DNA replication in Escherichia coli.
AUTOR(ES)
Xia, W
RESUMO
In vitro, anionic phospholipids can reactivate inactivated DnaA protein, which is essential for initiation of DNA replication at the oriC site of Escherichia coli [Sekimizu, K. & Kornberg, A. (1988) J. Biol. Chem. 263, 7131-7135]. Mutations in the pgsA gene (encoding phosphatidylglycerophosphate synthase) limit the synthesis of the major anionic phospholipids and lead to arrest of cell growth. We report herein that a mutation in the rnhA gene (encoding RNase H) that bypasses the need for the DnaA protein through induction of constitutive stable DNA replication [Kogoma, T. & von Meyenburg, K. (1983) EMBO J. 2, 463-468] also suppressed the growth arrest phenotype of a pgsA mutant. The maintenance of plasmids dependent on an oriC site for replication, and therefore DnaA protein, was also compromised under conditions of limiting anionic phospholipid synthesis. These results provide support for the involvement of anionic phospholipids in normal initiation of DNA replication at oriC in vivo by the DnaA protein.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=42704Documentos Relacionados
- Initiation of DNA replication in Escherichia coli.
- Cooperation of the prs and dnaA gene products for initiation of chromosome replication in Escherichia coli.
- Membrane attachment activates dnaA protein, the initiation protein of chromosome replication in Escherichia coli.
- Homologous recombination-dependent initiation of DNA replication from DNA damage-inducible origins in Escherichia coli.
- In vivo studies of DnaA binding to the origin of replication of Escherichia coli.