In Vivo Gas Exchange Measurement of the Site and Dynamics of Nitrate Reduction in Soybean1
AUTOR(ES)
Cen, Yan-Ping
FONTE
American Society of Plant Biologists
RESUMO
A gas analysis system was built to study the relationship between the reductant cost of NO3− assimilation and the measured rate of CO2 and O2 exchange in roots, leaves, and stems+ petioles of soybean (Glycine max L. Merr. cv Maple glen) plants. The measurements were used to calculate the diverted reductant utilization rate (DRUR = 4*[measured rate of CO2 + measured rate of O2], in moles of high-energy electron [e−] per gram per hour) in plants in the presence (N+) and absence (N−) of NO3−. The differences in DRUR between the N+ and N− treatments provided a measure of the NO3−-coupled DRUR of 25-d-old plants, whereas a 15NO3−-enriched nutrient solution was used to obtain an independent measure of the rate of NO3− assimilation. The measured reductant cost for the whole plant was 9.6 e− per N assimilated, a value within the theoretical range of four to 10 e− per N assimilated. The results predicted that shoots accounted for about 55% of the whole-plant NO3− assimilation over the entire day, with shoots dominating in the light, and roots in the dark. The gas analysis approach described here holds promise as a powerful, noninvasive tool to study the regulation of NO3− assimilation in plant tissue.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=166878Documentos Relacionados
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