In vivo incorporation of Drosophila H2a histone into mammalian chromatin.
AUTOR(ES)
Reeves, R
RESUMO
Hybrid prokaryotic/eukaryotic expression vectors have been used to introduce Drosophila histone genes into CV-1 African green monkey tissue culture cells. Transfection of CV-1 cells with Drosophila genes under the control of insect DNA promoter sequences results in low level expression of histone genes. On the other hand, when the Drosophila H2a gene is juxtaposed downstream from the long terminal repeat sequence of Rous sarcoma virus (RSV) expression of the insect gene is considerably more efficient; both 3' polyadenylated insect histone messenger RNA and putative Drosophila H2a histone protein can be readily detected in the transduced cells. Using this RSV/H2a vector, we have been able to demonstrate the presence of Drosophila H2a histone in monomer nucleosome preparations isolated from transfected CV-1 cells. These results suggest the feasibility of 'remodeling' cellular chromatin in vivo in precisely defined ways. The techniques described may be generally applicable to other genes coding for chromosomal proteins.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=325917Documentos Relacionados
- Silencing of Unpaired Chromatin and Histone H2A Ubiquitination in Mammalian Meiosis
- Histone H2A subtypes associate interchangeably in vivo with histone H2B subtypes.
- Histone H1o: its location in chromatin.
- Histone H1 deposition and histone-DNA interactions in replicating chromatin.
- A new class of histone H2A mutations in Saccharomyces cerevisiae causes specific transcriptional defects in vivo.
