In vivo interactions of the Drosophila Hairy and Runt transcriptional repressors with target promoters.
AUTOR(ES)
Jiménez, G
RESUMO
The Hairy and Runt pair-rule proteins regulate Drosophila segmentation by repressing transcription. To explore the ability of these proteins to function as promoter-bound regulators in vivo, we examined the effects of Hairy and Runt derivatives containing heterologous transcriptional activation domains (HairyAct and RunAct). Using this approach, we find that Hairy and Runt efficiently target such activation domains to specific segmentation gene promoters, leading to rapid induction of transcription. Our results strongly suggest that Hairy normally acts as a promoter-bound repressor of fushi tarazu, runt and odd-skipped, and that Runt directly represses even-skipped. We also show that expressing HairyAct in early blastoderm embryos causes ectopic Sex-lethal expression and male-specific lethality, implying that the Hairy-related denominator element Deadpan represses Sex-lethal during sex determination by directly recognizing the early Sex-lethal promoter.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=452534Documentos Relacionados
- Variations in transcriptional activity of rDNA spacer promoters.
- Nucleotide pool-sensitive selection of the transcriptional start site in vivo at the Salmonella typhimurium pyrC and pyrD promoters.
- Hairy Transcriptional Repression Targets and Cofactor Recruitment in Drosophila
- Transcriptional control mediated by the ArcA two-component response regulator protein of Escherichia coli: characterization of DNA binding at target promoters.
- Interactions of Drosophila Ultrabithorax Regulatory Regions with Native and Foreign Promoters