In vivo repair of the 3'terminus of transfer RNA injected into amphibian oocytes.
AUTOR(ES)
Solari, A
RESUMO
Yeast transfer RNA specific for phenylalanine has been treated chemically to remove either one or two nucleotides of its 3' terminus and has been injected into Xenopus laevis oocytes to test whether this RNA can be repaired in vivo. The results obtained showed that oocytes could aminoacylate and thus repair tRNAPhe that has lost both its terminal adenosine and 3' phosphate. A similar result was obtained with tRNAPhe that had undergone two full cycles of 3' terminal nucleotide removal. The oocytes cannot aminoacylate tRNAPhe whose 3' terminal ribose has been oxidized with periodate or the derivative that retains a 3' phosphate after adenosine removal. In vitro assays show that the Xenopus ovary contains a tRNA nucleotidyl transferase with the properties similar to enzymes obtained from other sources which may be responsible for the 3' terminal repair observed in vitro.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=342528Documentos Relacionados
- Gene activation in somatic nuclei after injection into amphibian oocytes.
- Coupled amplification and degradation of exogenous RNA injected in amphibian oocytes
- High-fidelity transcription of 5S DNA injected into Xenopus oocytes.
- Karyophilic proteins: polypeptides synthesized in vitro accumulate in the nucleus on microinjection into the cytoplasm of amphibian oocytes.
- Transcription of cloned Moloney murine leukemia proviral DNA injected into Xenopus laevis oocytes.
