In Vivo Splicing and Functional Characterization of Mycobacterium leprae RecA
AUTOR(ES)
Frischkorn, Klaus
FONTE
American Society for Microbiology
RESUMO
The RecA proteins from Mycobacterium tuberculosis and Mycobacterium leprae contain inteins. In contrast to the M. tuberculosis RecA, the M. leprae RecA is not spliced in Escherichia coli. We demonstrate here that M. leprae RecA is functionally spliced in Mycobacterium smegmatis and produces resistance toward DNA-damaging agents and homologous recombination.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=101971Documentos Relacionados
- Characterization of the Two Mycobacterium tuberculosis recA Promoters
- Protein splicing in trans by purified N- and C-terminal fragments of the Mycobacterium tuberculosis RecA intein
- Characterization of Mycobacterium leprae RecA Intein, a LAGLIDADG Homing Endonuclease, Reveals a Unique Mode of DNA Binding, Helical Distortion, and Cleavage Compared with a Canonical LAGLIDADG Homing Endonuclease*
- Functional Properties of Borrelia burgdorferi recA
- Molecular cloning and characterization of the recA gene of Rhizobium phaseoli and construction of recA mutants.
