Inactivation of Hydrogenase in Cell-free Extracts and Whole Cells of Chlamydomonas reinhardi by Oxygen 1
AUTOR(ES)
Erbes, David L.
RESUMO
O2 irreversibly inactivates hydrogenase from Chlamydomonas reinhardi. The mechanism for the inactivation involves the reaction of one molecule of hydrogenase with one molecule of O2 (or two oxygen atoms) in the transition complex of the rate-limiting step. The second order rate constant for this reaction is 190 atmospheres−1 minute−1 (1.4 × 105 molar−1 minute−1). At levels above 0.01 atmosphere O2, the increased numbers of O2 molecules may compete for the site of inactivation hindering the proper orientation for inactivation of any one O2 molecule and resulting in lowered rates of inactivation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=542984Documentos Relacionados
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