Incomplete and Aberrant Particles of Actinophage MSP2 from Lysates of Streptomyces venezuelae
AUTOR(ES)
Jones, Lily A.
RESUMO
Lysates of actinophage MSP2, propagated on Streptomyces venezuelae S13, contain at least 1011 PFU/ml. During purification by centrifugation methods and by adsorption chromatography, a number of types of aberrant and incomplete phage particles were seen by electron microscopy. Infectious MSP2 had a buoyant density in CsCl of 1.52 g/cm3 and an absorbance at 260 nm relative to that at 280 nm (A260/A280) of 1.53. Empty capsids banded at 1.276 g/cm3 and partially filled capsids banded at 1.351 g/cm3, and A260/A280 ratios were 0.77 and 1.24, respectively. Two kinds of light capsids found in CsCl fractions of 1.278 g/cm3 probably include the 1.276 component. Some capsids were joined by tail-like structures. Ghosts and polyheads also were present. Aberrant particles observed by electron microscopy included two-tailed actinophage, phage with abnormal tail positions, and large-headed phage.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=355542Documentos Relacionados
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