Increased mutagenesis mediated by cloned plasmid CAM-OCT genes: potential for expanding substrate ranges of Pseudomonas spp.
AUTOR(ES)
McBeth, D L
RESUMO
Twenty-five kilobases of Pseudomonas plasmid CAM-OCT DNA encoding a DNA repair gene(s) was cloned into the broad-host-range vector pVK100. The presence of the cloned genes increased the isolation frequency of Pseudomonas putida derivatives capable of using ethyl lactate or 3-methyl-3-buten-1-ol as their carbon source 15- and 8-fold, respectively, after UV irradiation. Ethyl lactate-utilizing strains expressed a novel intracellular hydrolase.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=168155Documentos Relacionados
- The CAM-OCT plasmid enhances UV responses of Pseudomonas aeruginosa recA mutants.
- Effect of degradative plasmid CAM-OCT on responses of Pseudomonas bacteria to UV light.
- Control potential of Meloidogyne javanica and Ditylenchus spp. using fluorescent Pseudomonas and Bacillus spp.
- Homologous structural genes and similar induction patterns in Azotobacter spp. and Pseudomonas spp.
- Diversidade e potencial biotecnológico de Pseudomonas spp. de sedimentos de manguezais.