Infection of SK-N-MC cells, a CD4-negative neuroblastoma cell line, with primary human immunodeficiency virus type 1 isolates.
AUTOR(ES)
Harouse, J M
RESUMO
Most studies looking at CD4-independent infection have used laboratory strains or their respective molecular clones. To determine whether primary human immunodeficiency virus type 1 isolates could infect CD4-negative cells, we obtained a panel of 23 clinical isolates and characterized the early steps of the viral life cycle in SK-N-MC cells, a CD4-negative, galactosylceramide-positive neuroblastoma cell line. Eight of 23 isolates established a nonproductive infection; entry and postentry restrictions were noted in the others. We were unable to correlate the infectivity for SK-N-MC cells with established biological phenotypes, such as syncytium induction, or with genetic classifications, suggesting that pantropism is an independent biological variable.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=190791Documentos Relacionados
- Human immunodeficiency virus type 1 infection of SK-N-MC cells: domains of gp120 involved in entry into a CD4-negative, galactosyl ceramide/3' sulfo-galactosyl ceramide-positive cell line.
- CD4-Negative Cells Bind Human Immunodeficiency Virus Type 1 and Efficiently Transfer Virus to T Cells
- Human immunodeficiency virus can infect CD4-negative human fibroblastoid cells.
- Primary Human Immunodeficiency Virus Type 2 (HIV-2) Isolates Infect CD4-Negative Cells via CCR5 and CXCR4: Comparison with HIV-1 and Simian Immunodeficiency Virus and Relevance to Cell Tropism In Vivo
- Human immunodeficiency virus type 2 infection and fusion of CD4-negative human cell lines: induction and enhancement by soluble CD4.