Influence of Effluent Irrigation on Community Composition and Function of Ammonia-Oxidizing Bacteria in Soil

AUTOR(ES)

Oved, Tamar

FONTE

American Society for Microbiology

RESUMO

The effect of effluent irrigation on community composition and function of ammonia-oxidizing bacteria (AOB) in soil was evaluated, using techniques of molecular biology and analytical soil chemistry. Analyses were conducted on soil sampled from lysimeters and from a grapefruit orchard which had been irrigated with wastewater effluent or fertilizer-amended water (FAW). Specifically, comparisons of AOB community composition were conducted using denaturing gradient gel electrophoresis (DGGE) of PCR-amplified fragments of the gene encoding the α-subunit of the ammonia monooxygenase gene (amoA) recovered from soil samples and subsequent sequencing of relevant bands. A significant and consistent shift in the population composition of AOB was detected in soil irrigated with effluent. This shift was absent in soils irrigated with FAW, despite the fact that the ammonium concentration in the FAW was similar. At the end of the irrigation period, Nitrosospira-like populations were dominant in soils irrigated with FAW, while Nitrosomonas-like populations were dominant in effluent-irrigated soils. Furthermore, DGGE analysis of the amoA gene proved to be a powerful tool in evaluating the soil AOB community population and population shifts therein.

Documentos Relacionados

• Community Structure of Ammonia-Oxidizing Bacteria within Anoxic Marine Sediments
• Urease-Encoding Genes in Ammonia-Oxidizing Bacteria†
• Quantification of Ammonia-Oxidizing Bacteria in Arable Soil by Real-Time PCR
• Competition between Ammonia-Oxidizing Bacteria and Benthic Microalgae
• The Pure Culture Isolation of Ammonia-oxidizing Bacteria