Influence of Nickel on the Detection of Nitrate Reductase Activity in Sorghum Extracts 1
AUTOR(ES)
Maranville, Jerry W.
RESUMO
The addition of nickel (4 × 10−3m) to the extracting buffer enhances the nitrate reductase activity in preparations of young grain sorghum (Sorghum bicolor L. [Moench] leaf tissue by as much as 6-fold. Activities comparable to other plant species are obtained over an extraction pH range of 7 to 8 with tris buffer and reduced nicotinamide adenine dinucleotide as a cofactor for the reaction when the ratio of plant material to extraction medium is 1:20. The method also enhances nitrate reductase activity in sudangrass (Sorghum sudanense P. [Stapf]).
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=396467Documentos Relacionados
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