Inhibition of Photosynthetic Carbon Dioxide Fixation in Isolated Spinach Chloroplasts Exposed to Reduced Osmotic Potentials 1

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Reduced osmotic potentials inhibited the rate of CO2 fixation by isolated intact spinach (Spinacia oleracea) chloroplasts. This inhibition was observed immediately after transfer of chloroplasts from a solution containing 0.33 m sorbitol to higher sorbitol concentrations, and the depressed rate remained constant. The inhibited CO2 fixation could not be attributed to a decreased rate of photosynthetic electron transport, since NADP reduction was unaffected by subjecting the chloroplasts to low potentials. It could also not result from restricted permeability to CO2, as CO2 concentrations had no effect on the relative inhibition induced by the lowered potential.

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