INHIBITION OF RIBONUCLEIC ACID SYNTHESIS IN NEWCASTLE DISEASE VIRUS-INFECTED CELLS BY PUROMYCIN AND 6-AZAURIDINE

AUTOR(ES)

Wilson, D. E.

RESUMO

Wilson, D. E. (Rensselaer Polytechnic Institute, Troy, N.Y.), and P. LoGerfo. Inhibition of ribonucleic acid synthesis in Newcastle disease virus-infected cells by puromycin and 6-azauridine. J. Bacteriol. 88:1550–1555. 1964.—Puromycin and 6-azauridine were used to inhibit protein and ribonucleic acid (RNA) synthesis during Newcastle disease virus replication. Viral RNA, measured by the incorporation of uridine-2-C14, increased rapidly 6 to 7 hr after infection and reached a peak at 12 hr. Viral RNA synthesis was inhibited by puromycin added at the time of infection or at any time up to 4 hr after infection, but not thereafter. 6-Azauridine (3 mg/ml) inhibited over 90% of the viral RNA synthesis and inhibited protein synthesis to a small extent. Infected cells synthesized more viral RNA than did untreated controls when exposed to azauridine alone for 5 hr, followed by puromycin alone for 9 hr. It was concluded that immediately after virus infection, virus-specific proteins, which are necessary for viral RNA synthesis, were synthesized. Virus-specific protein synthesis may occur even when viral RNA synthesis is greatly inhibited. After virus-specific protein synthesis has taken place, viral RNA synthesis may take place when protein synthesis is almost totally inhibited.

Documentos Relacionados

• Thymidine metabolism and DNA synthesis in Newcastle disease virus-infected cells.
• Protein Synthesis in Newcastle Disease Virus-Infected Chicken Embryo Cells
• Synthesis of Viral-Specific Ribonucleic Acid in Rubella Virus-Infected Cells
• Measles Virus Ribonucleic Acid and Protein Synthesis: Effects of 6-Azauridine and Cycloheximide on Viral Replication
• Selective inhibition of protein synthesis in virus-infected mammalian cells.