Initiation Factor IF-3-Dependent Binding of Escherichia coli Ribosomes and N-Formylmethionine Transfer-RNA to Rabbit Globin Messenger
AUTOR(ES)
Noll, Markus
RESUMO
An initiation complex has been formed in high yields from E. coli ribosomes, 9S messenger RNA for rabbit hemoglobin, and N-formylmethionine-tRNA. Initiation factor IF-3 is required for the binding and puromycin is required for the release of fMet. Valyl-tRNA fails to bind to the second codon, whereas a mixture of 15 aminoacyl-tRNAs promotes incorporation. Together with quantitative data, the findings suggest that IF-3 directs the ribosomes to an AUG codon on one of the two globin messengers, at a site that is different from the normal starting point for globin synthesis.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=426815Documentos Relacionados
- Further studies on the initiation of protein synthesis with N-formylmethionine in E. coli extracts.
- Arangement of transfer-RNA -genes in yeast.
- Mechanism of dissociation of ribosomes of Escherichia coli by initiation factor IF-3.
- Interaction of rabbit reticulocyte ribosomes with bacteriophage f1 mRNA and of Escherichia coli ribosomes with rabbit globin mRNA
- Initiation of protein synthesis. 3. Factor-GTP-codon-dependent binding of F-met-tRNA to ribosomes.
