Initiation of chromosome replication in Escherichia coli after induction of dnaA gene expression from a lac promoter.
AUTOR(ES)
Bremer, H
RESUMO
Escherichia coli HB282 carries a dnaA46(Ts) allele on the chromosome, a wild-type dnaA allele under the control of the lacUV5 promoter on the multicopy plasmid pBC32, and an overproducing lac repressor allele on an F' factor. When the plasmid dnaA gene is repressed, the strain is thermosensitive. After a temporary deficiency in active dnaA protein at nonpermissive temperature, the addition of isopropyl-beta-D-thiogalactopyranoside to the culture was found to produce a burst of initiations within 5 to 10 min at 30% of the origins in 90% of the cells. Initiations then continued at a rate slightly faster than the mass-doubling time such that after 2 h the origin-to-mass ratio of the control culture was restored.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=214341Documentos Relacionados
- Cooperation of the prs and dnaA gene products for initiation of chromosome replication in Escherichia coli.
- Coordination of chromosome replication initiation in Escherichia coli: effects of different dnaA alleles.
- Expression of accumulated capacity for initiation of chromosome and minichromosome replication in dnaA mutants of Escherichia coli.
- Membrane attachment activates dnaA protein, the initiation protein of chromosome replication in Escherichia coli.
- Initiation of chromosome replication in dnaA and dnaC mutants of Escherichia coli B/r F.
