Insertion of a rabbit beta-globin gene sequence into an E. coli plasmid.
AUTOR(ES)
Rougeon, F
RESUMO
Double stranded DNA has been synthesized in vitro from rabbit globin messenger RNA and elongated with homopolymeric dG tails. An E. coli plasmid was cleaved by EcoRI. The cohesive ends were repaired and dC tails added, to permit reconstitution of the EcoRI sites upon annealing with the dG elongated globin DNA. Transformation of E. coli with the globin-plasmid DNA hybrid has yielded a clone which harbours a recombinant plasmid (pCR1-betaG1), as demonstrated by hybridization experiments with radioactive globin cDNA. The sequence carried by the recombinant plasmid corresponds to part of the gene sequence coding for the beta chain of rabbit globin. Circular DNA of the purified recombinant plasmid exhibits sensitivity to EcoRI.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=343603Documentos Relacionados
- Integration of synthetic globin genes into an E. coli plasmid.
- Transformation of frog embryos with a rabbit beta-globin gene.
- Genomic sequence of rat beta-globin major gene.
- Genomic sequence of rat beta-globin minor gene.
- The intervening sequence of a mouse beta-globin gene is transcribed within the 15S beta-globin mRNA precursor.
