Interaction of a protein from rat liver nuclei with cruciform DNA.

AUTOR(ES)

Bianchi, M E

RESUMO

We constructed a synthetic cruciform DNA which closely resembles Holliday junctions, a DNA structure formed during recombination or following the transition from interstrand to intrastrand base pairing in palindromic DNA sequences. We identified and partially purified a protein from rat liver that specifically binds to this cruciform DNA molecule and does not bind to single-stranded or double-stranded DNAs of the same sequence. This protein also binds to the cruciform structure formed by a 70 bp palindromic sequence cloned in plasmid pUC18. No detectable nucleolytic activity is associated with the rat liver cruciform-binding protein, in contrast to all cruciform-recognizing proteins known so far.

Documentos Relacionados

• Single-strand DNA binding protein from rat liver: interactions with supercoiled DNA.
• A novel type of interaction between cruciform DNA and a cruciform binding protein from HeLa cells.
• Studies of the interaction of RecA protein with DNA.
• Slow cruciform transitions in palindromic DNA.
• Interaction of bleomycin with DNA.