Interaction of human peripheral blood monocytes with apoptotic polymorphonuclear cells

AUTOR(ES)

Mikołajczyk, Tomasz P

FONTE

Blackwell Science Inc

RESUMO

Macrophages have the potential to recognize apoptotic neutrophils and phagocytose them while the same function for monocytes is uncertain. In fact, early findings indicated that monocytes started to phagocytose neutrophils on the third day of differentiation to macrophages. Here we show, using flow cytometry and confocal microscopy, that peripheral blood monocytes phagocytose apoptotic but not freshly isolated granulocytes. Recognition of apoptotic cells is predominantly connected with CD16+ monocytes (CD14high CD16+ and CD14dim CD16+) and requires CD36. Clearance of apoptotic polymorphonuclear leucocytes appears to be independent of the CD14 mechanism. Uptake of apoptotic Jurkat T cells by monocytes is CD14 and CD36 dependent. Liposomes containing phosphatidyl-l-serine reduce binding of apoptotic polymorphonuclear leucocytes. Lipopolysaccharide-activated subpopulations of monocytes while in contact with apoptotic cells produce more anti-inflammatory cytokine interleukin-10 whereas the production of pro-inflammatory cytokines, tumour necrosis factor-α and interleukin-1β is reduced.

Documentos Relacionados

• Induction of Apoptotic Cell Death in Peripheral Blood Mononuclear and Polymorphonuclear Cells by an Oral Bacterium, Fusobacterium nucleatum
• Interaction of influenza A virus with human peripheral blood lymphocytes.
• Multi-nucleated giant cell formation from human cord blood monocytes in vitro, in comparison with adult peripheral blood monocytes
• The Binding of Human Immunoglobulin G1 Monomer and Small, Covalently Cross-Linked Polymers of Immunoglobulin G1 to Human Peripheral Blood Monocytes and Polymorphonuclear Leukocytes
• Interaction of leptospires with human polymorphonuclear neutrophils.