Interaction of Phalloidin with Actin

AUTOR(ES)

Lengsfeld, Anneliese M.

RESUMO

Phalloidin, a toxic bicyclic peptide of rapid action from the toadstool, Amanita phalloides, gives rise to polymerization of G-actin to filamentous structures (Ph-actin) in a medium of low ionic strength. Ph-actin closely resembles the microfilaments found in liver membrane fractions (Ph-filaments) after in vivo or in vitro poisoning. Both phalloidin induced filaments are resistant to 0.6 M KI in contrast to F-actin, and become decorated by heavy meromyosin. After preincubation with cytochalasin B significantly fewer actin filaments are observed.

Documentos Relacionados

• The phalloidin binding site of F-actin.
• Cytoskeletal F-actin patterns quantitated with fluorescein isothiocyanate-phalloidin in normal and transformed cells.
• Phalloidin-induced actin polymerization in the cytoplasm of cultured cells interferes with cell locomotion and growth
• Interaction of filamin with f-actin in solution.
• Interaction of a Dictyostelium member of the plastin/fimbrin family with actin filaments and actin-myosin complexes.