Interaction of purified lipoteichoic acid with the classical complement pathway.

AUTOR(ES)

Loos, M

RESUMO

Glycerophosphate-containing lipoteichoic acids (LTAs) interact with the first component of the classical complement pathway (C1). This resulted in the activation of the classical complement pathway in serum, shown by the consumption of C1, C2, and C4. The dose-dependent interaction of LTAs with purified C1 and C1q was dependent on the negative charges of the phosphate groups of LTA. It was reduced by charge compensation through D-alanine ester substituents and by sterical hindrance through di- and trihexosyl residues linked to position 2 of the glycerol moieties. The charge density of LTA may also play a role: poly(digalactosylglycerophosphate) LTAs, in which the phosphate groups are in a greater distance from each other, were less effective, and the loss of micellar organization by deacylation of LTA drastically reduced the complement activation capacity.

Documentos Relacionados

• Legionella pneumophila lipopolysaccharide activates the classical complement pathway.
• Plasmid-associated resistance of Salmonella typhimurium to complement activated by the classical pathway.
• Activation of the alternative complement pathway by pneumococcal lipoteichoic acid.
• Role of immunoglobulin G in killing of Borrelia burgdorferi by the classical complement pathway.
• Requirements for the solubilization of immune aggregates by complement. The role of the classical pathway.