Interaction of the Operator of the Tryptophan Operon with Repressor
AUTOR(ES)
Rose, John K.
RESUMO
Transcription studies in vitro on repression of the tryptophan operon of Escherichia coli show that partially purified trp repressor binds specifically to DNA containing the trp operator with a repressor-operator dissociation constant of about 0.2 nM in 0.12 M salt at 37°, a value consistent with the extent of trp operon regulation in vivo. The half-life of the trp repressor-trp operator complex is less than 2 min in vitro in 0.12 M salt.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=388636Documentos Relacionados
- The interaction of the recognition helix of lac repressor with lac operator.
- Interaction of Gal repressor with inducer and operator: Induction of gal transcription from repressor-bound DNA
- lac repressor-lac operator interaction: NMR observations.
- Regulatory elements of the raffinose operon: nucleotide sequences of operator and repressor genes.
- Interaction of the Bacillus subtilis glnRA repressor with operator and promoter sequences in vivo.