Intercalation of psoralen into DNA of plastid chromosomes decreases late during barley chloroplast development.
AUTOR(ES)
Davies, J P
RESUMO
We have used a DNA crosslinking assay to measure intercalation of the psoralen derivative HMT (4'-hydroxymethyl-4,5',8-trimethylpsoralen) into barley (Hordeum vulgare) plastid chromosomal DNA during chloroplast and etioplast development. Intercalation into DNA in intact plastids in vivo and in plastid lysates in vitro shows that chromosomal DNA in the most mature chloroplasts intercalates HMT less efficiently than DNA in younger chloroplasts. In contrast, there is no change in HMT intercalation during etioplast differentiation in the dark. Our results also show that DNA in higher plant plastid chromosomes is under superhelical tension in vivo. The lower susceptibility to HMT intercalation of DNA in the most mature chloroplasts indicates that late during chloroplast development the superhelical tension or the binding of proteins to the DNA or both change.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=328879Documentos Relacionados
- Plastid Transcription Activity and DNA Copy Number Increase Early in Barley Chloroplast Development 1
- The expression of nuclear genes encoding plastid ribosomal proteins precedes the expression of chloroplast genes during early phases of chloroplast development.
- Identification of plastid envelope proteins required for import of protochlorophyllide oxidoreductase A into the chloroplast of barley
- Changes in Chloroplast mRNA Stability during Leaf Development.
- Organization of the Light-Harvesting Complex of Photosystem I and Its Assembly during Plastid Development.