Interchain hydrogen-bonding interactions may facilitate translocation of K+ ions across the potassium channel selectivity filter, as suggested by synthetic modeling chemistry
AUTOR(ES)
Mareque Rivas, Juan C.
FONTE
National Academy of Sciences
RESUMO
A 4-fold symmetric arrangement of TVGYG polypeptides forms the selectivity filter of the K+ channel from Streptomyces lividans (KcsA). We report the synthesis and properties of synthetic models for the filter, p-tert-butyl-calix[4]arene-(OCH2CO-XOBz)4 (X = V, VG, VGY), 1–3. The first cation (Na+, K+) binds to the four -{OCH2CO}- units, a region devised to mimic the metal-binding site formed by the four T residues in KcsA. NMR studies reveal that cations and valine amide protons compete for the carbonyl oxygen atoms, converting N—HVal⋅⋅⋅O⩵C hydrogen bonds to M+⋅⋅⋅O⩵C bonds (M+ = Na+ or K+). The strength of these interchain N—HVal⋅⋅⋅O⩵C hydrogen bonds varies in the order 3 > 2 > 1. We propose that such interchain H-bonding may destabilize metal binding in the selectivity filter and thus help create the low energy barrier needed for rapid cation translocation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=55477Documentos Relacionados
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