Interferon increases the abundance of submembranous microfilaments in HeLa-S3 cells in suspension culture.

AUTOR(ES)

Wang, E

RESUMO

Human beta (fibroblast) interferon inhibits the proliferation of human HeLa-S3 carcinoma cells in suspension culture. Accompanying this effect, the lateral mobility of cell surface receptors for concanavalin A is decreased and the rigidity of the plasma membrane lipid bilayer is increased. The present findings show a marked increase in the number of polymerized actin-containing microfilaments 3 days after treatment of HeLa-S3 cells with beta-interferon (640 units/ml). The cortical region of the treated enlarged cells contains a thick and dense meshwork of 40-70 A microfilaments. The actin nature of the filaments was verified by their ability to bind heavy meromyosin. These results support the concept that beta-interferon induces a coordinated response in the plasma membrane and the underlying microfilaments in both tumor and normal cells.

Documentos Relacionados

• Interferon stimulates cholesterol and phosphatidylcholine synthesis but inhibits cholesterol ester synthesis in HeLa-S3 cells.
• Bordetella parapertussis invasion of HeLa 229 cells and human respiratory epithelial cells in primary culture.
• Thyrotropin preparations are mitogenic for thyroid epithelial cells in follicles in suspension culture.
• Cytological subdivision of the S phase of human cells in asynchronous culture.
• Hypoosmotic Shock Induces Increases in Cytosolic Ca2+ in Tobacco Suspension-Culture Cells.