Intermediates of adeno-associated virus DNA replication in vitro.
AUTOR(ES)
Hong, G
RESUMO
Intermediates of adeno-associated virus type 2 (AAV) DNA replication in an in vitro assay have been characterized. The assay involves rescue and replication of an AAV insert in pBR322. Intermediates were shown to be duplex molecules in which at least one terminus was in the extended configuration, in contrast to the hairpinned ends seen after rescue in the absence of AAV DNA replication. Also present were linear double-stranded dimers, which were characterized as either head-to-head or tail-to-tail tandems; no head-to-tail dimers were detected. The results are in accord with the current model of AAV DNA replication.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=236668Documentos Relacionados
- In vitro replication of adeno-associated virus DNA.
- In vitro replication of adeno-associated virus DNA.
- In Vitro Packaging of Adeno-Associated Virus DNA
- Role of the Adenovirus DNA-Binding Protein in In Vitro Adeno-Associated Virus DNA Replication
- Genetic analysis of adeno-associated virus: properties of deletion mutants constructed in vitro and evidence for an adeno-associated virus replication function.
