Intracellular distribution of terminal deoxynucleotidyl transferase in rat bone marrow and thymus
AUTOR(ES)
Gregoire, Kathleen E.
RESUMO
A subset of bone marrow cells that contains terminal deoxynucleotidyl transferase (DNA nucleotidylexotransferase; nucleosidetriphosphate:DNA deoxynucleotidylexotransferase, EC 2.7.7.31) can be identified in adult rats by immunofluorescence using affinity-column-purified antibody to homogeneous calf transferase. The transferase-positive cells comprise approximately 1.8% of bone marrow cells. Correcting the specific activity of terminal transferase in total bone marrow cells (0.21 units per 108 cells) for the percentage of transferase-positive bone marrow cells (1.8%) gives 11.7 units per 108 cells, a value approximately half that found for transferase-positive thymocytes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=431815Documentos Relacionados
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