Intracellular transcription of G-rich DNAs induces formation of G-loops, novel structures containing G4 DNA
AUTOR(ES)
Duquette, Michelle L.
FONTE
Cold Spring Harbor Laboratory Press
RESUMO
We show that intracellular transcription of G-rich regions produces novel DNA structures, visible by electron microscopy as large (150–500 bp) loops. These G-loops are formed cotranscriptionally, and they contain G4 DNA on one strand and a stable RNA/DNA hybrid on the other. G-loop formation requires a G-rich nontemplate strand and reflects the unusual stability of the rG/dC base pair. G-loops and G4 DNA form efficiently within plasmid genomes transcribed in vitro or in Escherichia coli. These results establish that G4 DNA can form in vivo, a finding with implications for stability and maintenance of all G-rich genomic regions.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=443523Documentos Relacionados
- Reduced aggregation and improved specificity of G-rich oligodeoxyribonucleotides containing pyrazolo[3,4-d]pyrimidine guanine bases
- Structure of Long Telomeric RNA Transcripts: THE G-RICH RNA FORMS A COMPACT REPEATING STRUCTURE CONTAINING G-QUARTETS*
- Cell cycle-regulated generation of single-stranded G-rich DNA in the absence of telomerase
- Normal human chromosomes have long G-rich telomeric overhangs at one end
- Isolation of cDNA encoding a binding protein specific to 5'-phosphorylated single-stranded DNA with G-rich sequences.