Intramolecular transposition by a synthetic IS50 (Tn5) derivative.
AUTOR(ES)
Tomcsanyi, T
RESUMO
We report the formation of deletions and inversions by intramolecular transposition of Tn5-derived mobile elements. The synthetic transposons used contained the IS50 O and I end segments and the transposase gene, a contraselectable gene encoding sucrose sensitivity (sacB), antibiotic resistance genes, and a plasmid replication origin. Both deletions and inversions were associated with loss of a 300-bp segment that is designated the vector because it is outside of the transposon. Deletions were severalfold more frequent than inversions, perhaps reflecting constraints on DNA twisting or abortive transposition. Restriction and DNA sequence analyses showed that both types of rearrangements extended from one transposon end to many different sites in target DNA. In the case of inversions, transposition generated 9-bp direct repeats of target sequences.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=526819Documentos Relacionados
- Fis plays a role in Tn5 and IS50 transposition.
- Integration host factor plays a role in IS50 and Tn5 transposition.
- Identification of base pairs in the outside end of insertion sequence IS50 that are needed for IS50 and Tn5 transposition.
- Orientation of IS50 transposase gene and IS50 transposition.
- Induction of the SOS response in Escherichia coli inhibits Tn5 and IS50 transposition.