Intranuclear degradation of nonsense codon-containing mRNA
AUTOR(ES)
Bühler, Marc
FONTE
Oxford University Press
RESUMO
Most vertebrate mRNAs with premature termination codons (PTCs) are specifically recognized and degraded by a process referred to as nonsense-mediated mRNA decay (NMD) while still associated with the nucleus. However, it is still a matter of debate whether PTCs can be identified by intranuclear scanning or only by ribosomes on the cytoplasmic side of the nuclear envelope. Here we show that inhibition of mRNA export by two independent approaches does not affect the downregulation of PTC-containing T-cell receptor β transcripts in the nuclear fraction of mammalian cells, providing strong evidence for intranuclear NMD. Our results are fully consistent with recently reported evidence for nuclear translation and suggest that an important biological role for nuclear ribosomes is the early elimination of nonsense mRNA during a pioneer round of translation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1084183Documentos Relacionados
- Recognition of Yeast mRNAs as “Nonsense Containing” Leads to Both Inhibition of mRNA Translation and mRNA Degradation: Implications for the Control of mRNA Decapping
- Nonsense codons in human beta-globin mRNA result in the production of mRNA degradation products.
- Infrequent Translation of a Nonsense Codon Is Sufficient to Decrease mRNA Level
- Introns are cis effectors of the nonsense-codon-mediated reduction in nuclear mRNA abundance.
- Nonsense-Mediated Decay of Human HEXA mRNA