Introduction of the 3-Deoxy Group in 3,6-Dideoxyhexoses: A Novel Cofactor Function for Pyridoxamine-5′-Phosphate

AUTOR(ES)

Gonzalez-Porque, Pedro

RESUMO

A cofactor needed for the reduction of CDP-4-keto-6-deoxy-D-glucose to CDP-4-keto-3,6-dideoxy D-glucose has been detected and purified. Its spectra in neutral, acid, and basic solutions, its behavior on thin-layer chromatography, and other properties led to its identification as pyridoxamine-5′-phosphate. Moreover, authentic pyridoxamine-5′-phosphate substituted for the purified cofactor with about the same specific activity. Other vitamin B6 derivatives did not stimulate the reaction, and inhibition was observed with some of them.

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