Inverse transposition by the RAG1 and RAG2 proteins: role reversal of donor and target DNA
AUTOR(ES)
Shih, I-hung
FONTE
Oxford University Press
RESUMO
The lymphoid-specific proteins RAG1 and RAG2 initiate V(D)J recombination by introducing DNA double-strand breaks at the recombination signal sequences (RSSs). In addition to DNA cleavage, the versatile RAG1/2 complex is capable of catalyzing several other reactions, including hybrid joint formation and the transposition of signal ends into a second DNA. Here we show that the RAG1/2 complex also mediates an unusual strand transfer reaction, inverse transposition, in which non-RSS DNA is cleaved and subsequently transferred to an RSS sequence by direct transesterification. Characterization of the reaction products and requirements suggests that inverse transposition is related to both hybrid joint formation and signal-end transposition. This aberrant activity provides another possible mechanism for some chromosomal translocations present in lymphoid tumors.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=136934Documentos Relacionados
- DNA Hairpin Opening Mediated by the RAG1 and RAG2 Proteins
- Regulation of RAG1/RAG2-mediated transposition by GTP and the C-terminal region of RAG2
- Distinct Roles of RAG1 and RAG2 in Binding the V(D)J Recombination Signal Sequences
- DNA mismatches and GC-rich motifs target transposition by the RAG1/RAG2 transposase
- Mutational analysis of RAG1 and RAG2 identifies three catalytic amino acids in RAG1 critical for both cleavage steps of V(D)J recombination
