Involvement of folic acid and methionine in the synthesis of certain membrane-associated nucleotide sugars by Enterococcus hirae.

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RESUMO

A membrane preparation from Enterococcus hirae contained UDP and methyl-UDP (mUDP) monosaccharides. This preparation, when incubated with combinations of folic acid, L-serine, and S-adenosyl-L-methionine in a reaction system containing UDP-glucose, promoted synthesis of certain mUDP sugars. Folic acid and serine produced mUDP-glucose and mUDP-rhamnose; S-adenosylmethionine produced mUDP-glucose, mUDP-mannose, and mUDP-fucose.

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