Involvement of functional protein kinase C in the mitogenic response to the H-ras oncogene product.
AUTOR(ES)
Lacal, J C
RESUMO
Microinjection of purified protein kinase C (PKC) into Swiss 3T3 fibroblasts pretreated with the phorbol ester phorbol-12,13-dibutyrate restores the mitogenic response of the cells to phorbol-12,13-dibutyrate (G. Pasti, J.C. Lacal, B.S. Warren, S.A. Aaronson, and P.M. Blumberg, Nature [London] 324:375-377, 1986). Our present studies demonstrate that the mitogenic activity of the H-ras oncogene in H-ras p21-microinjected quiescent cells is markedly reduced under conditions in which PKC is downregulated by chronic phorbol ester treatment. The ability to reconstitute the mitogenic response upon microinjection of both H-ras p21 and PKC implies involvement of functional PKC in the mitogenic activity of the H-ras oncogene product.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=368090Documentos Relacionados
- Cells that overproduce protein kinase C are more susceptible to transformation by an activated H-ras oncogene.
- Novel revertants of H-ras oncogene-transformed R6-PKC3 cells.
- Reversible abrogation of IL-3 dependence by an inducible H-ras oncogene.
- Total chemical synthesis of a functional interacting protein pair: The protooncogene H-Ras and the Ras-binding domain of its effector c-Raf1
- Identification of residues of the H-ras protein critical for functional interaction with guanine nucleotide exchange factors.