Involvement of Two Protein Factors and ATP in In Vitro DNA Synthesis Catalyzed by DNA Polymerase III of Escherichia coli
AUTOR(ES)
Hurwitz, Jerard
RESUMO
The in vitro conversion of single-stranded DNA from bacteriophage fd to duplex structures depends on E. coli RNA polymerase, DNA polymerase III, riboand deoxyribonucleoside triphosphates, Mg+2, spermidine or DNA-unwinding protein of E. coli, and two additional protein factors, referred to here as Factors I and II. These two factors are also essential for dTMP incorporation catalyzed by DNA polymerase III and dependent on poly(dA)·oligo(dT) primer-template. In the latter reaction, there is an absolute dependency on ATP or dATP.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=387920Documentos Relacionados
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