Ion etching of human adenovirus 2: structure of the core.
AUTOR(ES)
Newcomb, W W
RESUMO
The surface of human adenovirus 2 was etched by irradiating intact virions with low-energy (1-keV) Ar+ ions in a Technics Hummer V sputter coater . Viral structures exposed by the etching process were shadowed and then examined in the electron microscope. Periods of etching that were sufficient to reduce the viral diameter by 20 to 30 nm revealed distinct substructural elements in the virion core. Cores were found to consist of a cluster of 12 large, uniformly size spheres which abutted one another in the intact virion. The spheres, for which we suggest the name " adenosomes ," had a diameter of 23.0 +/- 2.3 nm, and they were related to each other by two-, three-, and fivefold axes of rotational symmetry. The results support the view, originally suggested by Brown et al. (J. Virol. 16:366-387, 1975) that the adenovirus 2 core is composed of 12 large spheres packed tightly together in such a way that each is directed toward the vertex of an icosahedron . Such a structure, constructed of 23.0-nm-diameter spheres, would have an outside diameter (vertex-to-vertex distance) of 67.0 nm and a face-to-face distance of 58.2 nm. It could be accommodated inside the icosahedral adenovirus capsid if each large sphere were located beneath a capsid vertex.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=254398Documentos Relacionados
- Structure and composition of the adenovirus type 2 core.
- Structure of human plasma low-density lipoproteins: molecular organization of the central core.
- Structural organization and polypeptide composition of the avian adenovirus core.
- The helical model of the nucleosome core.
- Reconstruction of the three-dimensional structure of simian virus 40 and visualization of the chromatin core.