IS231A from Bacillus thuringiensis is functional in Escherichia coli: transposition and insertion specificity.
AUTOR(ES)
Hallet, B
RESUMO
A kanamycin resistance gene was introduced within the insertion sequence IS231A from Bacillus thuringiensis, and transposition of the element was demonstrated in Escherichia coli. DNA sequencing at the target sites showed that IS231A transposition results in direct repeats of variable lengths (10, 11, and 12 bp). These target sequences resemble the terminal inverted repeats of the transposon Tn4430, which are the preferred natural insertion sites of IS231 in B. thuringiensis.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=208117Documentos Relacionados
- Integrated Physical and Genetic Mapping of Bacillus cereus and Other Gram-Positive Bacteria Based on IS231A Transposition Vectors
- Mechanism of mutation by thymine starvation in Escherichia coli: clues from mutagenic specificity.
- Nucleotide sequence and structural organization of an insertion sequence element (IS231) from Bacillus thuringiensis strain berliner 1715.
- Seryl-tRNA synthetase from Escherichia coli: implication of its N-terminal domain in aminoacylation activity and specificity.
- Mechanism of Is1 Transposition in E. COLI: Choice between Simple Insertion and Cointegration